CST, 10521SF, ATF-2/ATF-7 (A9G1M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATF7/ATF-2. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, ATF-2/ATF-7 (A9G1M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #10521) is ready to ship. Product Usage Information This product is the carrier free version of product #82870. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 82870 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ATF-2/ATF-7 (A9G1M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ATF-2 and ATF-7 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ATF-2 protein. Background The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). ATF-7 is another member of the ATF/CREB family of leucine zipper proteins (5). Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions (6,7). Alternate Names Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; Activating transcription factor 7; ATF-2; ATF2; ATF7; ATFA; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-7; cAMP-responsive element-binding protein 2; CRE-BP1; CREB-2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-7; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MGC57182; Transcription factor ATF-A; TREB7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65, 75 Source/Isotype: Rabbit IgG