CST, 10775W2, Phospho-Stat5 (Tyr694) (D47E7) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 700 Conjugate)

Monoclonal Antibody for studying STAT5A (Tyr694) phosphate/STAT5B (Tyr699) phosphate. Highly specific and rigorously validated in-house, Phospho-Stat5 (Tyr694) (D47E7) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 700 Conjugate) (CST #10775) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-Stat5 (Tyr694) (D47E7) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein. Background Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Alternate Names epididymis secretory sperm binding protein; GHISID2; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5A; STA5B; STAT5; STAT5A; STAT5B; transcription factor STAT5B Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG