CST, 11815T, ATF-4 (D4B8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATF-4. Validated for WB,IP,IF,ChIP,ChIP,C&R,C&T. Available in 3 sizes. Highly specific and rigorously validated in-house, ATF-4 (D4B8) Rabbit Monoclonal Antibody (CST #11815) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using the CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Chromatin IP: 1:200 Chromatin IP-seq: 1:200 CUT&RUN: 1:200 CUT&Tag: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 82093 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity ATF-4 (D4B8) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-4 protein. Reactivity by immunofluorescence is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein. Background ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4). Alternate Names Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG