CST, 11817S, Phospho-GRB10 (Ser476) (D4E6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Grb10 (Ser476) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-GRB10 (Ser476) (D4E6) Rabbit Monoclonal Antibody (CST #11817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-GRB10 (Ser476) (D4E6) Rabbit Monoclonal Antibody recognizes endogenous levels of GRB10 protein only when phosphorylated at Ser476. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser476 of human GRB10 protein. Background The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4). Recently GRB10 was shown to be a direct substrate of mTORC1 (5). It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation (5). Alternate Names Grb-10; GRB-IR; GRB10; GRB10 adapter protein; GRB10 adaptor protein; GRBIR; growth factor receptor bound protein 10; Growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; IRBP; KIAA0207; maternally expressed gene 1; MEG1; RSS Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG