CST, 11891T, CHD8 (D3C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHD8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHD8 (D3C1) Rabbit Monoclonal Antibody (CST #11891) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHD8 (D3C1) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHD8 protein. This antibody also cross-reacts with a protein of unknown origin at 140 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CHD8 protein. Background CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10). Alternate Names ATP-dependent helicase CHD8; AUTS18; axis duplication inhibitor; beta-catenin binding protein-like; CHD-8; CHD8; chromodomain helicase DNA binding protein 8; Chromodomain-helicase-DNA-binding protein 8; DKFZp686N17164; duplin; Helicase with SNF2 domain 1; HELSNF1; KIAA1564 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 290 Source/Isotype: Rabbit IgG