CST, 11943S, OTX2 (D7Y3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OTX2. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, OTX2 (D7Y3J) Rabbit Monoclonal Antibody (CST #11943) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity OTX2 (D7Y3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total OTX2 protein. This antibody recognizes both known human isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn251 of human OTX2 protein. Background Orthodenticle homeobox 2 (OTX2) belongs to the bicoid subfamily of paired-box, homeodomain-containing transcription factors. OTX2 is a critically important neuronal transcription factor that functions to regulate the expression of cell cycle genes controlling proliferation and differentiation of neural progenitor cells (1-3). In addition to its neuronal development functions, it has been reported that OTX2 can function in a non-cell autonomous manner to promote survival of damaged retinal ganglion cells (4). OTX2 has also been shown to influence the susceptibility of post-mitotic neurons to toxic insult or physiological stress (3). Notably, aberrant expression of OTX2 has been strongly linked with neuronal tumor development. For example, research studies have found OTX2 is overexpressed in many medulloblastoma cell lines, and both overexpression and gene amplification were reported in a subset of primary medulloblastomas (5). studies support these observations, as targeted alterations in OTX2 expression directly affected both proliferation and senescence of medulloblastoma cell lines (6,7). Alternate Names CPHD6; Homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; Orthodenticle homolog 2; OTX2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 31, 33 Source/Isotype: Rabbit IgG