CST, 11957S, ADAP Antibody

Polyclonal Antibody for studying ADAP. Validated for Western Blotting. Highly specific and rigorously validated in-house, ADAP Antibody (CST #11957) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ADAP Antibody recognizes endogenous levels of total ADAP protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro731 of human ADAP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background ADAP (adhesion and degranulation-promoting adaptor protein/SLAP-130/Fyb) is an SH3 domain-containing adaptor protein expressed by T cells, NK cells, and myeloid cells (1,2). There are two isoforms of ADAP with predicted molecular weights of 85 kDa and 90 kDa, but observed molecular weights of 120 kDa and 130 kDa (1-3). ADAP was identified as an adaptor protein that interacts with SLP-76 following T cell receptor (TCR) stimulation and was subsequently found to be important for several aspects of T cell activation (1,2). For example, ADAP is required for integrin-dependent clustering, signaling, and adhesion (4,5). In addition, ADAP interacts with CARMA1 and facilitates assembly of the CARMA1-Bcl10-MALT1 complex important for NF-κB activation downstream of TCR activation (6). Finally, following binding of a T cell to an antigen presenting cell, ADAP forms a ring at the immunological synapse that recruits dynein to enable microtubule-organizing center polarization (7). Alternate Names ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130; FYB1; FYN binding protein (FYB-120/130); FYN binding protein 1; FYN-binding protein; FYN-binding protein (FYB-120/130); FYN-binding protein 1; FYN-T-binding protein; p120/p130; PRO0823; SLAP-130; SLAP130; SLP-76-associated phosphoprotein; THC3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 120, 130 SOURCE: Rabbit