CST, 11966S, BRM (D9E8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BRM. Validated for WB,WB,IP,IHC,IF,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, BRM (D9E8B) Rabbit Monoclonal Antibody (CST #11966) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 48277 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity BRM (D9E8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRM protein. This antibody does not cross-react with BRG1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly264 of human BRM protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13). Alternate Names ATP-dependent helicase SMARCA2; BAF190; BAF190B; brahma homolog; BRG1-associated factor 190B; BRM; FLJ36757; global transcription activator homologous sequence; hBRM; hSNF2a; MGC74511; NCBRS; Probable global transcription activator SNF2L2; Protein brahma homolog; SMARCA2; SMCA2; SNF2; SNF2-alpha; SNF2-like 2; SNF2/SWI2-like protein 2; SNF2A; SNF2L2; SNF2LA; Sth1p; sucrose nonfermenting 2-like protein 2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SWI2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG