CST, 11976S, Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NPY. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody (CST #11976) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77100 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody recognizes endogenous levels of precursor (11 kDa) and mature (4 kDa) neuropeptide Y. This antibody does not cross-react with peptide YY or peptide YY (3-36) peptides. This antibody may cross-react with a protein of unknown origin at 80 kDa in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human mature neuropeptide Y. Background Neuropeptide Y (NPY) is a 36 amino acid peptide that belongs to the pancreatic polypeptide (PP) family, which also includes peptide YY (PYY) (1). The mature 36-residue NPY is produced from a larger pre-pro 97-residue NPY precursor through a series of cleavage reactions at dibasic sites and C-terminal amidation of the peptide product (2). NPY is widely expressed in the central nervous system (3) and exerts its action through stimulation of 5 different receptors (Y1-Y5) that belong to the G protein-coupled receptor family (4). NPY in the hypothalamus exhibits orexigenic activity through activation of Y1 and Y5 receptors (5). NPY is involved in the control of bone homeostasis, through the regulation of osteoblast activity by Y1 and Y2 receptors (6), and the regulation of testosterone secretion by activating Y1 receptor in testicular vessels (7). Research studies suggest that modulation of NPY activity and signaling represents a potential strategy for the development of appetite control and antiobesity agents (8). Alternate Names C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y; NPY; prepro-neuropeptide Y; Pro-neuropeptide Y; PYY4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 4, 11 Source/Isotype: Rabbit IgG