CST, 11984T, beta-TrCP (D12C8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying beta-Trcp. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, beta-TrCP (D12C8) Rabbit Monoclonal Antibody (CST #11984) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta-TrCP (D12C8) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-TrCP1 protein. This antibody does not detect β-TrCP2 (FBW1B). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human β-TrCP protein. Background β-transducin repeat-containing protein (β-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1,2). β-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IκBα, β-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of β-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6). Alternate Names beta-transducin repeat containing; beta-transducin repeat containing E3 ubiquitin protein ligase; BETA-TRCP; beta-TrCP1; betaTrCP; BTRC; BTRCP; bTrCP1; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; F-box and WD-repeat protein 1B; F-box/WD repeat-containing protein 1A; FBW1A; FBXW1; FBXW1A; FWD1; MGC4643; pIkappaBalpha-E3 receptor subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG