CST, 11994S, Phospho-DDR1 (Tyr792) Antibody

Polyclonal Antibody for studying DDR1 (Tyr792) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-DDR1 (Tyr792) Antibody (CST #11994) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-DDR1 (Tyr792) Antibody recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr792. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr792 of human DDR1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1). Phosphorylation of DDR1 at Tyr792 was identified at Cell Signaling Technology using PTMScan , our LC-MS/MS platform for phosphorylation site discovery (3). Tyr792 is located in the activation loop of the DDR1 kinase domain. Alternate Names CAK; CD167; CD167 antigen-like family member A; CD167a; Cell adhesion kinase; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EDDR1; Epithelial discoidin domain receptor 1; Epithelial discoidin domain-containing receptor 1; HGK2; mammarian carcinoma kinase 10; Mammary carcinoma kinase 10; MCK-10; MCK10; NEP; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; NTRK4; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; PTK3; PTK3A; PTK3A protein tyrosine kinase 3A; RTK6; TRK E; TRKE; Tyrosine kinase DDR; Tyrosine-protein kinase CAK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 125 SOURCE: Rabbit