CST, 12005T, Phospho-REPS1 (Ser709) (D8C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying REPS1 (Ser709) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-REPS1 (Ser709) (D8C1) Rabbit Monoclonal Antibody (CST #12005) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-REPS1 (Ser709) (D8C1) Rabbit Monoclonal Antibody recognizes endogenous levels of REPS1 protein only when phosphorylated at Ser709. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser709 of human REPS1 protein. Background REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified. Phosphorylation of REPS1 at Ser709 was identified at Cell Signaling Technology using PTMScan Technology, our LC-MS/MS platform for phosphorylation site discovery (4). Alternate Names NBIA7; RALBP1; RALBP1 associated Eps domain containing 1; RalBP1-associated Eps domain-containing protein 1; RalBP1-interacting protein 1; REPS1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG