CST, 12040T, UPF1 (D15G6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UPF1. Validated for WB,IP,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, UPF1 (D15G6) Rabbit Monoclonal Antibody (CST #12040) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UPF1 (D15G6) Rabbit Monoclonal Antibody recognizes endogenous levels of total UPF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UPF1 protein. Background UPF1 was identified as an active component in nonsense-mediated mRNA decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). UPF1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). UPF1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, UPF2 and UPF3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5). Alternate Names ATP-dependent helicase RENT1; delta helicase; FLJ43809; FLJ46894; hUpf1; KIAA0221; Nonsense mRNA reducing factor 1; NORF1; pNORF1; Regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; UP Frameshift 1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1; UPF1 regulator of nonsense transcripts homolog; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1 RNA helicase and ATPase; yeast Upf1p homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG