CST, 12100S, Aurora A (1F8) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Aurora A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Aurora A (1F8) Mouse Monoclonal Antibody (CST #12100) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Aurora A (1F8) Mouse Monoclonal Antibody recognizes endogenous levels of total Aurora A protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human Aurora A protein. Background Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6). Alternate Names AIK; AIRK1; ARK-1; ARK1; AURA; AURKA; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; hARK1; IAK1; IPL1-related kinase; MGC34538; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK6; STK7 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Mouse IgG1