CST, 12110S, Phospho-MERIT40 (Ser29) Antibody

Polyclonal Antibody for studying NBA1 (Ser29) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-MERIT40 (Ser29) Antibody (CST #12110) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-MERIT40 (Ser29) Antibody recognizes endogenous levels of MERIT40 protein only when phosphorylated at Ser29. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser29 of human MERIT40 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair. BRCA1 forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for the regulation of BRCA1 function. The BRCA1-Rap80 complex (BRCA1 A complex), including Rap80, BRCC36, BRCC45, Abraxas, and MERIT40/NBA1, functions in G2/M phase checkpoint control (reviewed in 1,2). MERIT40/NBA1 localizes to sites of DNA damage and is required for the appropriate localization of BRCA1 in response to ionizing radiation, as well as maintenance of the BRCA1 A complex (3,4). Proteomics studies have identified Ser29 as a phosphorylated site on MERIT40/NBA1, and the significance of this phosphorylation is under investigation (5-9). Alternate Names BABA1; BABAM1; BRCA1-A complex subunit MERIT40; BRISC and BRCA1 A complex member 1; BRISC and BRCA1-A complex member 1; C19orf62; chromosome 19 open reading frame 62; FLJ20571; HSPC142; mediator of Rap80 interactions and targeting 40 kDa; Mediator of RAP80 interactions and targeting subunit of 40 kDa; MERIT40; MRI40; NBA1; New component of the BRCA1-A complex; new component of the BRCAA1 A complex Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit