CST, 12120S, Bcl-11B (D6F1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bcl-11B. Validated for WB,IP,IF,IF,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Bcl-11B (D6F1) Rabbit Monoclonal Antibody (CST #12120) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 16148 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity Bcl-11B (D6F1) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bcl-11B protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein. Background Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C H type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2 and 3 zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9). Alternate Names ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BAF chromatin remodeling complex subunit BCL11B; BC11B; BCL-11B; BCL11B; BCL11B, BAF complex component; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP-2; CTIP2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; Radiation-induced tumor suppressor gene 1 protein; RIT1; zinc finger protein hRit1 alpha; ZNF856B Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 120-130 Source/Isotype: Rabbit IgG