CST, 12137S, Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POU3F2/Brn2. Validated for WB,IP,IF,ChIP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody (CST #12137) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:1600 - 1:3200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35173 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Brn2/POU3F2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro97 of human Brn2/POU3F2 protein. Background Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8). Alternate Names Brain-2; Brain-specific homeobox/POU domain protein 2; Brn-2; BRN2; N-Oct3; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; OTF7; PO3F2; POU class 3 homeobox 2; POU domain, class 3, transcription factor 2; POU3F2; POUF3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG