CST, 12159S, LLGL1 (D2B5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LLGL1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, LLGL1 (D2B5A) Rabbit Monoclonal Antibody (CST #12159) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LLGL1 (D2B5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total LLGL1 protein. This antibody does not cross-react with LLGL2. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu757 of human LLGL1 protein. Background In , lethal giant larvae ( ), discs large ( ), and scribble ( ) act as tumor suppressor genes. Their loss of function in flies causes neoplastic overgrowth of larval brain tissue and imaginal epithelial cells hallmarked by disruption of the cytoskeletal network and cellular polarity (1,2). The human homolog of the protein, lethal giant larvae protein homolog 1 (LLGL1), is a cytoskeletal protein implicated in regulating cellular organization, migration, and cell polarity (3). As in , decreased expression of LLGL1 correlates with an increased incidence of cellular overgrowth and malignant transformation (4-6). In mammalian epithelial cells, LLGL1 redistributes from the cytoplasm to regions of cell-cell contact, allowing the establishment and maintainence of a polarized morphology (7). LLGL1 also plays a role in the formation of epithelial junctions via its direct interactions with PAR6 and aPKC, the latter of which has been shown to phosphorylate LLGL1 at Ser663, thus restricting its localization to the basolateral region of the cell (8). LLGL1 may also play an additional, unrealized role in cellular development and differentiation as indicated by the fact that has been implicated in controlling self-renewal and differentiation of progenitor cells (9). Recent studies in mice have suggested that a mammalian LLGL1 homolog that does not have tumor suppressor-like acitvity, LLGL2, is required for proper polarized invasion of trophoblasts and efficient branching morphogenesis during placental development (10). Alternate Names DLG4; HUGL; Hugl-1; HUGL1; Human homolog to the D-lgl gene protein; L2GL1; lethal giant larvae homolog 1 (Drosophila); lethal giant larvae homolog 1, scribble cell polarity complex component; Lethal(2) giant larvae protein homolog 1; Lgl1; LLGL; LLGL scribble cell polarity complex component 1; LLGL1; LLGL1, scribble cell polarity complex component; Mgl1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG