CST, 12166S, MAML1 (D3K7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAML1. Validated for WB,IP,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, MAML1 (D3K7B) Rabbit Monoclonal Antibody (CST #12166) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq Specificity / Sensitivity MAML1 (D3K7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAML1 protein. This antibody does not detect MAML2 or MAML3. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp269 of human MAML1 protein. Background Mastermind-like (MAML) family of proteins are homologs of Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7). Alternate Names FLJ53540; KIAA0200; Mam-1; Mam1; MAML1; mastermind homolog; mastermind like transcriptional coactivator 1; mastermind-like 1; mastermind-like 1 (Drosophila); Mastermind-like protein 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG