CST, 12218S, SHANK2 Antibody

Polyclonal Antibody for studying SHANK2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SHANK2 Antibody (CST #12218) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (1). Alternate Names AUTS17; cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CORTBP1; CTTNBP1; GKAP/SAPAP interacting protein; KIAA1022; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; PROSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHAN2; SHANK; SHANK2; SHANK2 iso4; SPANK-3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 165 SOURCE: Rabbit