CST, 12219S, PTMScan® Trypsin Digested Control Peptides I

Cell Extracts for studying in the research area. Product Usage Information PTMScan ® Trypsin Digested Control Peptides I are supplied as lyophilized powder. Centrifuge the tube containing the lyophilized peptide in order to collect all material to be dissolved. Add 1.4 ml PTMScan ® IAP Buffer (1X) and follow the PTMScan ® kit protocol from the Immunoaffinity Purification (IAP) step using the entire vial of reconstituted peptides. Storage Store lyophilized peptides at -20°C. In lyophilized form, the peptides are stable for 24 months. Background PTMScan Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan ), ubiquitination (UbiScan ), acetylation (AcetylScan ), and methylation (MethylScan ), among others. PTMScan Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). Alternate Names PTMScan