CST, 12243S, Drebrin A Antibody

Polyclonal Antibody for studying Drebrin. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Drebrin A Antibody (CST #12243) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Drebrin A Antibody recognizes endogenous levels of total Drebrin A protein. This antibody does not cross-react with Drebrin E. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala330 of mouse Drebrin A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Developmentally-regulated brain proteins (Drebrins) are cytoplasmic proteins that were originally identified in the brain as F-actin-binding proteins. There are two mammalian isoforms: adult type (A) and embryonic type (E). These isoforms are derived from a single gene through alternative RNA splicing mechanisms (1). Drebrin E has been observed to accumulate in the developmental stage of migrating neurons and in the growing cell processes of neurons. Drebrin A is found at the dendritic spines of mature cortical neurons, where it plays a role in synaptic plasticity (2,3). Although Drebrins are primarily found in neurons, they have also been found in skeletal muscle, heart, pancreas, and kidney. Research studies have shown that reduced expression of Drebrin in the brain could be associated with Alzheimer's Disease, Down Syndrome (4), and bipolar disorders (5). Alternate Names D0S117E; DBN1; Developmentally-regulated brain protein; DKFZp434D064; DREB; Drebrin; drebrin 1; drebrin A; drebrin E; drebrin E2; drebrin-1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 132 SOURCE: Rabbit