CST, 12263T, G6PD (D5D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying G6PD. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, G6PD (D5D2) Rabbit Monoclonal Antibody (CST #12263) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity G6PD (D5D2) Rabbit Monoclonal Antibody recognizes endogenous levels of total G6PD protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human G6PD protein. Background Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first, and rate-limiting, step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Research studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2). Alternate Names epididymis secretory sperm binding protein; G6PD; G6PD1; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG