CST, 12280T, Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MKK3 (Ser218) phosphate/MKK6 (Ser207) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit Monoclonal Antibody (CST #12280) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73317 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit Monoclonal Antibody recognizes endogenous levels of MKK3 and MKK6 proteins only when phosphorylated at Ser189 (MKK3) or Ser207 (MKK6). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein. Background MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5). Alternate Names Dual specificity mitogen-activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 3; MAP kinase kinase 6; MAP2K3; MAP2K6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; MEK6; mitogen-activated protein kinase kinase 3; mitogen-activated protein kinase kinase 6; MKK3; MKK6; MP2K3; MP2K6; PRKMK3; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; SAPKK-2; SAPKK-3; SAPKK2; SAPKK3; SKK2; SKK3; Stress-activated protein kinase kinase 2; Stress-activated protein kinase kinase 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 38 MKK6, 40 MKK3 Source/Isotype: Rabbit IgG