CST, 12282S, Cox2 (D5H5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cyclooxygenase-2/Cox-2. Validated for WB,WB,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Cox2 (D5H5) Rabbit Monoclonal Antibody (CST #12282) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73315 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cox2 (D5H5) Rabbit Monoclonal Antibody recognizes endogenous levels of total Cox2 protein. Species cross-reactivity for IF-IC is mouse and rat only. Non-specific labeling of centrioles may be observed by immunofluorescence in human cells. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His108 of human Cox2 protein. Background Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6). Alternate Names COX-2; COX2; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGH2; PGHS-2; PHS II; PHS-2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); PTGS2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 74 Source/Isotype: Rabbit IgG