CST, 12291S, Protein A (HRP Conjugate)

Miscellaneous for studying in the research area. Product Usage Information This product should be used in place of the secondary in the western blotting protocol. Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Protocol Available protocols: Western Blotting Specificity / Sensitivity Species Reactivity: All Species Expected Source / Purification Protein A (HRP Conjugate) is purified by gel filtration. The conjugate product is free from unconjugated protein A and HRP. Background Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and therefore can be used for detection and/or purification of antibodies. Due to its vast array of applications, protein A is the method of choice for many preparative and analytical immunological assays (1).