CST, 12293T, ATP2A1/SERCA1 (D54G12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATP2A1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ATP2A1/SERCA1 (D54G12) Rabbit Monoclonal Antibody (CST #12293) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ATP2A1/SERCA1 (D54G12) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATP2A1/SERCA1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro995 of human ATP2A1/SERCA1 protein. Background Sarcoplasmic and endoplasmic reticulum Ca ATPases (SERCA) are members of a highly conserved family of Ca pumps (1). SERCA pumps transport Ca from the cytosol to the sarcoplasmic and endoplasmic reticulum lumen against a large concentration gradient (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum Ca ATPase (2). Research studies have shown that mutations in the ATP2A1 gene cause an autosomal recessive muscle disorder known as Brody myopathy, which is characterized by muscle cramping and impaired muscle relaxation associated with exercise (1-3). Alternate Names AT2A1; ATP2A; ATP2A1; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, fast twitch 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG