CST, 12339S, TPH-1 (D10C10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TPH1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TPH-1 (D10C10) Rabbit Monoclonal Antibody (CST #12339) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TPH-1 (D10C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total TPH-1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TPH-1 protein. Background Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the gene results in low levels of circulating and tissue serotonin (4). Alternate Names indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; TPH1; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG