CST, 12382S, AUF1/hnRNP D (D6O4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP D0. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, AUF1/hnRNP D (D6O4F) Rabbit Monoclonal Antibody (CST #12382) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity AUF1/hnRNP D (D6O4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total AUF1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AUF1 protein. Background AU-rich element RNA binding protein 1 (AUF1) is also known as heterogeneous ribonucleoprotein D (hnRNP D). AUF1 binds to the AU rich element (ARE) of target mRNA and regulates mRNA decay (1,2). It has a broad range of target genes including IL-1, IL-2, IL-3, Myc, TNF-α, and cyclin D1 (2). Binding of AUF1 to Myc mRNA also affects translation of Myc (3). Recent studies have provided evidence that AUF1 is also involved in the regulation of transcription. AUF1 binds to the promoters of various genes including complement receptor 2 (4), enkephalin (5), and α-fetoprotein (6). AUF1 also binds to the telomerase catalytic subunit Tert promoter and the G-rich telomeric repeat, thus regulating telomere maintenance and normal aging (7,8). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (9,10). All AUF1 isoforms shuttle between the nucleus and cytoplasm (11, 12). These isoforms have distinct localization and bind to different target mRNAs that contribute to the diversity of AUF1 function (2). Alternate Names ARE-binding protein AUFI, type A; AU-rich element RNA binding protein 1, 37kDa; AU-rich element RNA-binding protein 1; AUF1; AUF1A; heterogeneous nuclear ribonucleoprotein D; heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa); Heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; HNRNPD; hnRNPD0; HNRPD; P37 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37-48 Source/Isotype: Rabbit IgG