CST, 12412S, PAX3 Antibody

Polyclonal Antibody for studying PAX3. Validated for Western Blotting. Highly specific and rigorously validated in-house, PAX3 Antibody (CST #12412) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PAX3 Antibody recognizes transfected levels of total PAX3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PAX3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). Alternate Names CDHS; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3; transcriptional factor PAX3; WS1; WS3 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 62 SOURCE: Rabbit