CST, 12426S, IL-1 beta (D4T2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL1 beta mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-1 beta (D4T2D) Rabbit Monoclonal Antibody (CST #12426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-1 beta (D4T2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse IL-1β protein. This antibody can detect 500 pg of mature recombinant mouse IL-1β. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val175 of mouse IL-1β protein. Background Interleukin-1β (IL-1β) is a proinflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP); the complex then binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII, which lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3). Alternate Names IL-; IL-1 beta; Il-1b; IL-1beta; Il1b; interleukin 1 beta; Interleukin-1 beta; OTTMUSP00000016525 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 17,31 Source/Isotype: Rabbit IgG