CST, 12436T, FIP200 (D10D11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RB1CC1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FIP200 (D10D11) Rabbit Monoclonal Antibody (CST #12436) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FIP200 (D10D11) Rabbit Monoclonal Antibody recognizes endogenous levels of total FIP200 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FIP200 protein. Background FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members (1). FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation (2). FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration (reviewed in 3). FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation (4). FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy (5-7). Alternate Names 200 kDa FAK family kinase-interacting protein; ATG17; CC1; FAK family kinase-interacting protein of 200 kDa; FIP200; KIAA0203; phosphatase 1, regulatory subunit 131; PPP1R131; RB1 inducible coiled-coil 1; RB1-inducible coiled-coil 1; RB1-inducible coiled-coil protein 1; RB1CC1; RBCC1; RBICC Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG