CST, 12450S, Puma (D30C10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PUMA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Puma (D30C10) Rabbit Monoclonal Antibody (CST #12450) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Puma (D30C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total Puma protein. This antibody also cross-reacts with a protein of unknown origin at 60 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Puma protein. Background Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). Alternate Names BBC3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; BCL2 binding component 3; FLJ42994; JFY-1; JFY1; p53 up-regulated modulator of apoptosis; p53-upregulated modulator of apoptosis; PUMA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG