CST, 12454S, VDAC (D73D12) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying VDAC1. Validated for Western Blotting. Highly specific and rigorously validated in-house, VDAC (D73D12) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #12454) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VDAC (D73D12) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total VDAC protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human VDAC-1 protein. Background Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5). Alternate Names hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; PORIN; Porin 31HL; Porin 31HM; PORIN-31-HL; sperm binding protein 1a; VDAC; VDAC-1; VDAC1; voltage dependent anion channel 1; voltage-dependent anion channel 1; Voltage-dependent anion-selective channel protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG