CST, 12475S, beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate)

Monoclonal Antibody for studying Catenin-beta. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate) (CST #12475) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate) recognizes endogenous levels of total β-catenin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein Background β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Alternate Names armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 92 Source/Isotype: Rabbit IgG