CST, 12483T, SMARCA1 (D4Q7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMARCA1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMARCA1 (D4Q7V) Rabbit Monoclonal Antibody (CST #12483) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMARCA1 (D4Q7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMARCA1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro73 of human SMARCA1 protein. Background SMARCA1 (SNF2L) is one of the two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). The ISWI chromatin remodeling complexes were first identified in and have been shown to remodel and alter nucleosome spacing (2). SMARCA1 is the catalytic subunit of the nucleosome remodeling factor (NURF) and CECR2-containing remodeling factor (CERF) complexes (3-5). The NURF complex plays an important role in neuronal physiology by promoting neurite outgrowth and regulation of homeotic genes that are involved in neuronal development in the mid-hindbrain (3). NURF is also thought to be involved in the maturation of T cells from thymocytes by regulating chromatin structure and expression of genes important for T cell development (6). The largest subunit of the NURF complex, BPTF, is required for proper development of mesoderm, endoderm, and ectoderm tissue lineages, suggesting a role for SMARCA1 in the development of the germ layers in mouse embryo (7). Disruption of the CERF complex by deletion of CECR2, an interacting partner of SMARCA1, is associated with the neural tube defect exencephaly, linking the CERF complex with regulation of neurulation (4). Alternate Names ATP-dependent helicase SMARCA1; DKFZp686D1623; FLJ41547; global transcription activator homologous sequence; hSNF2L; ISWI; Nucleosome-remodeling factor subunit SNF2L; NURF140; Probable global transcription activator SNF2L1; SMARCA1; SMCA1; SNF2-like 1; SNF2L; SNF2L1; SNF2LB; SNF2LT; sucrose nonfermenting 2-like protein 1; SWI; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1; SWI2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG