CST, 12486T, SirT6 (D8D12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SirT6 mouse. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SirT6 (D8D12) Rabbit Monoclonal Antibody (CST #12486) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 40431 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SirT6 (D8D12) Rabbit Monoclonal Antibody recognizes endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length mouse SirT6 protein. Background The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H O (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2). Alternate Names 2810449N18Rik; AI043036; NAD-dependent deacetylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; NAD-dependent protein deacylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; Sir2l6; SIR6; Sirt6; sirtuin 6; sirtuin 6 (silent mating type information regulation 2, homolog) 6 (S. cerevisiae) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42, 36 Source/Isotype: Rabbit IgG