CST, 12542S, Jak3 (D1H3) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Jak3. Validated for Western Blotting. Highly specific and rigorously validated in-house, Jak3 (D1H3) Rabbit Monoclonal Antibody (Biotinylated) (CST #12542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Jak3 (D1H3) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total Jak3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Jak3 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-3; JAK3; JAK3_HUMAN; JAKL; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L-JAK; Leukocyte janus kinase; LJAK; Tyrosine-protein kinase JAK3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit IgG