CST, 12551S, mGluR1 (D5H10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying mGluR1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, mGluR1 (D5H10) Rabbit Monoclonal Antibody (CST #12551) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 95008 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity mGluR1 (D5H10) Rabbit Monoclonal Antibody recognizes endogenous levels of total mGluR1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1105 of human mGluR1 protein. Background Metabotropic glutamate receptor 1 (mGluR1) is a G protein-coupled receptor (GPCR) for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). Both mGluR1 and mGluR5 are coupled to phospholipase C and activate inositol phospholipid metabolism via G protein-mediated mechanisms. Upon phosphatidylinositol activation, the second messenger calcium is released and generates a calcium-activated chloride current. Metabotropic glutamate receptors other than mGluR1 and mGluR5 inhibit adenylate cyclase (1-3). mGluR1 does not share sequence homology with conventional GPCRs (1). mGluR1 forms a homodimer and is linked to synaptic plasticity, as well as long-term potentiation and long-term depression. Furthermore, mGluR1 is a potential therapeutic target for various psychiatric and neurological diseases, including schizophrenia, epilepsy, and Parkinson and Alzheimer diseases (4-6). Alternate Names glutamate metabotropic receptor 1; glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1A; Metabotropic glutamate receptor 1; mGlu1; MGLUR1; MGLUR1A; PPP1R85; protein phosphatase 1, regulatory subunit 85; SCA44; SCAR13 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 145, >300 Source/Isotype: Rabbit IgG