CST, 12552S, c-Raf (D5X6R) Mouse Monoclonal Antibody

Monoclonal Antibody for studying c-Raf. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, c-Raf (D5X6R) Mouse Monoclonal Antibody (CST #12552) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity c-Raf (D5X6R) Mouse Monoclonal Antibody recognizes endogenous levels of total c-Raf protein. This antibody does not cross-react with A-Raf or B-Raf. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues in the middle of human c-Raf protein. Background A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser446), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser365, Ser429, and Thr440) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11). Alternate Names c-Raf; C-Raf proto-oncogene, serine/threonine kinase; CMD1NN; cRaf; NS5; Oncogene RAF1; Proto-oncogene c-RAF; RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; Raf-1 proto-oncogene, serine/threonine kinase; RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1 Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Mouse IgG1