CST, 12573S, LIN28A (D1A1A) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying LIN28A. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, LIN28A (D1A1A) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #12573) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LIN28A (D1A1A) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein. Background LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). Alternate Names CSDD1; FLJ12457; LIN-28; lin-28 homolog A; lin-28 homolog A (C. elegans); Lin-28A; LIN28; LIN28A; LN28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; ZCCHC1; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG