CST, 12574S, DAG Lipase beta (D4P7C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DAGLBETA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DAG Lipase beta (D4P7C) Rabbit Monoclonal Antibody (CST #12574) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DAG Lipase beta (D4P7C) Rabbit Monoclonal Antibody recognizes endogenous levels of total DAG Lipase β protein. In some tissues, this antibody may detect a 48 kDa protein of unknown origin. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human DAG Lipase β protein. Background Diacylglycerol (DAG) lipases comprise two enzymes called DAG lipase α and β, which are the products of two related genes (1). DAG lipases are transmembrane proteins composed of a short amino-terminal intracellular domain, four transmembrane domains, and a large carboxy-terminal cytoplasmic domain containing the active site. These enzymes are responsible for the biosynthesis of 2-acylglycerol from diacylglycerol in a calcium-dependent manner (1). One of the major endocannabinoid ligands that activate cannabinoid receptors, 2-arachidonyl glycerol (2-AG), is produced by DAG lipases (2). Research studies suggest that DAG lipase α is the isoform primarily responsible for the central production of 2-AG (3). DAG lipase β has been implicated in studies of 2-AG production at the periphery in specific cell types and pathophysiological contexts, such as in hepatic stellate cells during alcohol induced fatty liver (4). Alternate Names DAGL-beta; DAGLB; DAGLBETA; DGL-beta; DGLB; diacylglycerol lipase beta; Diacylglycerol lipase-beta; diacylglycerol lipase, beta; FLJ33624; FLJ33909; FLJ45532; KCCR13L; PUFA-specific triacylglycerol lipase; Sn1-specific diacylglycerol lipase beta Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG