CST, 12586S, Lamin B1 (D4Q4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lamin B1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Lamin B1 (D4Q4Z) Rabbit Monoclonal Antibody (CST #12586) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Lamin B1 (D4Q4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total lamin B1 protein. This antibody recognizes the 25 kDa lamin B1 amino terminal cleavage product produced during apoptosis. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu118 of human lamin B1 protein. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene . Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10). Alternate Names ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 68, 25 Source/Isotype: Rabbit IgG