CST, 12665S, PANK4 (D6J4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PANK4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PANK4 (D6J4R) Rabbit Monoclonal Antibody (CST #12665) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PANK4 (D6J4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PANK4 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro522 of human PANK4 protein. Background Panthothenate kinase (PANK) is an enzyme that is responsible for catalyzing the first step in coenzyme A (CoA) synthesis (1-4). There are four human PANK genes (PANK1-4) (1-4). PANK4 is ubiquitously expressed, but higher expression levels are observed in muscle (1,2). PANK4 expression is elevated in rat skeletal muscle under high glucose conditions (2). There is evidence that rat PANK4 colocalizes with pyruvate kinase M2 (PKM2) (2). PANK4 may also play a protective role in beta-cell apoptosis by lowering the levels of pro-caspase-9 (3). Research studies have shown that mutations in the PANK2 gene are associated with Neurodegeneration with Brain Iron Accumulation (NBIA), formerly known as Hallervorden-Spatz syndrome (1,4). Expression of hPANK4 in a Drosophila model of NBIA rescues the phenotype with the exception of infertility (4). Alternate Names 4'-phosphopantetheine phosphatase; DKFZp547M242; FLJ10782; hPanK4; Inactive pantothenic acid kinase 4; PANK4; Pantothenate kinase 4; pantothenate kinase 4 (inactive); Pantothenic acid kinase 4 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG