CST, 12673S, RAD21 (D5Y8S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAD21. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RAD21 (D5Y8S) Rabbit Monoclonal Antibody (CST #12673) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RAD21 (D5Y8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total RAD21 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp213 of human RAD21 protein. Background The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Alternate Names 64-kDa C-terminal product; 64-kDa carboxy-terminal product; 65-kDa carboxy-terminal product; CDLS4; Double-strand-break repair protein rad21 homolog; FLJ25655; FLJ40596; hHR21; HR21; HRAD21; KIAA0078; kleisin; MCD1; MGS; Nuclear matrix protein 1; NXP-1; NXP1; protein involved in DNA double-strand break repair; RAD21; RAD21 cohesin complex component; RAD21 homolog; RAD21 homolog (S. pombe); SCC1; SCC1 homolog; sister chromatid cohesion 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit