CST, 12686T, Phospho-MCM3 (Ser112) (D3S4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MCM3 (Ser112) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MCM3 (Ser112) (D3S4M) Rabbit Monoclonal Antibody (CST #12686) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-MCM3 (Ser112) (D3S4M) Rabbit Monoclonal Antibody recognizes endogenous levels of MCM3 protein only when phosphorylated at Ser112. This antibody also recognizes a 200 kDa band of unknown origin in both interphase and mitotic cells. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of human MCM3 protein. Background The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10). Alternate Names cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3; MCM3 minichromosome maintenance deficient 3; MGC1157; minichromosome maintenance complex component 3; minichromosome maintenance deficient 3; P1-MCM3; P1.h; p102; replication licensing factor, beta subunit; RLF subunit beta; RLFB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG