CST, 12688S, His-Tag (D3I1O) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, His-Tag (D3I1O) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #12688) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity His-Tag (D3I1O) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes recombinant proteins containing the 6xHis epitope tag. This antibody conjugate recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the 6xHis epitope tag. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG