CST, 12697S, beta-Arrestin 1 (D8O3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Arrestin, beta1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, beta-Arrestin 1 (D8O3J) Rabbit Monoclonal Antibody (CST #12697) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity beta-Arrestin 1 (D8O3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-arrestin 1 protein. This antibody does not cross-react with β-arrestin 2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β-arrestin 1 protein. Background Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). A research study has shown that non-visual β-arrestins respond to glucocorticoid signaling, with differential responses observed among family members. Specifically, β-arrestin 1 expression is increased in response to glucocorticoid receptor activation whereas β-arrestin 2 shows a concomitant decrease in expression (11). Alternate Names ARB1; ARR1; ARRB1; arrestin 2; arrestin beta 1; Arrestin beta-1; arrestin, beta 1; beta-arrestin; Beta-arrestin-1; Non-visual arrestin-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG