CST, 12706S, DAZ1 Antibody

Polyclonal Antibody for studying DAZ1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DAZ1 Antibody (CST #12706) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DAZ1 Antibody recognizes endogenous levels of total DAZ1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro311 of human DAZ1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The human Deleted in AZoospermia (DAZ) genes encode a family of RNA-binding proteins that are expressed exclusively in the germ cells and play a role in the regulation of mRNA translation (1). Members of the family include DAZ, DAZL, and BOULE. The essential role of DAZL and BOULE in spermatogenesis has been established (2-4), but the role of DAZ remains unclear. Men with deletions in the DAZ1 gene can produce sperm, although in lower numbers, and are capable of reproduction and transmission of the genetic lesions (5-7). Alternate Names DAZ; DAZ1; deleted in azoospermia 1; Deleted in azoospermia protein 1; FLJ17175; SPGY; testicular tissue protein Li 49 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 SOURCE: Rabbit