CST, 12728S, Atlastin-1 (D2E6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying atlastin. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Atlastin-1 (D2E6) Rabbit Monoclonal Antibody (CST #12728) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Atlastin-1 (D2E6) Rabbit Monoclonal Antibody recognizes endogenous levels of total atlastin-1 protein. This antibody does not cross-react with atlastin-2 or atlastin-3. This antibody may cross-react with a protein of unknown origin at 100 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human atlastin-1 protein. Background Atlastin proteins are highly conserved members of the dynamin superfamily of membrane GTPases that are involved in the formation of vesicles for the endocytotic and secretory processes (1). Atlastins are required to establish and maintain the morphology of the tubular endoplasmic reticulum (ER) and are therefore important in ER function (2). GTP hydrolysis and dimerization are required for atlastin-dependent ER membrane fusion (3). Atlastin GTPase 1 (ATL1) is primarily expressed in brain, while the related atlastin 2 and atlastin 3 proteins are ubiquitously expressed (4). Mutations in the atlastin 1 gene SPG3A and the ER defects that result are thought to cause one form of hereditary spastic paraplegia (HSP), a group of heterogeneous neurological disorders characterized by severe progressive spasticity of the lower limbs (5,6). Alternate Names AD-FSP; ATL1; ATLA1; atlastin; atlastin GTPase 1; Atlastin-1; atlastin1; Brain-specific GTP-binding protein; FSP1; GBP-3; GBP3; GTP-binding protein 3; Guanine nucleotide-binding protein 3; guanylate-binding protein 3; hGBP3; HSN1D; Spastic paraplegia 3 protein A; SPG3; SPG3A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG